Technical Data

039620
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_057564.3; NP_001028284.1
Gene ID
51247
Gene #
PAIP2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PAIP2, NT (PAIP2, PAIP2A, Polyadenylate-binding protein-interacting protein 2)
PAIP2, PAIP2A, Polyadenylate-binding protein-interacting protein 2

PAIP2 acts as a repressor in the regulation of translation initiation of poly(A)-containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. Displaces the interaction of PABPC1 with poly(A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly(A) RNP structure organization.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PAIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human PAIP2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Graubert, T.A., et al. PLoS ONE 4 (2), E4583 (2009) :|Berlanga, J.J., et al. RNA 12(8):1556-1568(2006)|Yoshida, M., et al. EMBO J. 25(9):1934-1944(2006)|Onesto, C., et al. J. Biol. Chem. 279(33):34217-34226(2004)|Gouyon, F., et al. Biochem. J. 375 (PT 1), 167-174 (2003) :
USBio References
No references available
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