Technical Data

039703
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Accession #
NP_001595.2; NP_001121084.1
Gene ID
5080
Gene #
PAX6
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PAX6 (PAX6, AN2, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin)
PAX6, AN2, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq].

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PAX6 antibody is generated from rabbits immunized with a recombinant protein from human PAX6.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Gosmain, Y., et al. J. Biol. Chem. 285(43):33381-33393(2010)|Vuzman, D., et al. Biophys. J. 99(4):1202-1211(2010)|Zhang, X., et al. Cell Stem Cell 7(1):90-100(2010)|Bremond-Gignac, D., et al. Mol. Vis. 16, 1705-1711 (2010) :|Cai, F., et al. Mol. Vis. 16, 1141-1145 (2010) :
USBio References
No references available
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