039714
Clone Type
PolyclonalHost
RabbitSource
MouseIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
MoAccession #
NP_001035806.1; NP_000911.2Gene ID
5091Gene #
PCShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PC, NT (PC, Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase)
PC, Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq].
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-82 amino acids from the N-terminal region of human PC.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
MacDonald, M.J., et al. Diabetologia 52(6):1087-1091(2009)|Monnot, S., et al. Hum. Mutat. 30(5):734-740(2009)|Wang, D., et al. Mol. Genet. Metab. 95 (1-2), 31-38 (2008) :|Vora, S., et al. Natl Med J India 21(3):116-119(2008)|Xiang, S., et al. Nat. Struct. Mol. Biol. 15(3):295-302(2008)USBio References
No references available