Rabbit Anti-PCAT1, CT (LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-like 2, Phosphonoformate immuno-associated protein 3) (FITC)
LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-like 2, Phosphonoformate immuno-associated protein 3
PCAT1 acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to hosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis.
Applications
Suitable for use in FLISA, Western Blot and Immunohistochemisty. Other potential application, though not tested is Direct Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin fixed, paraffin embedded tissues Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 505-534 C-terminal region of human PCAT1.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human PCAT1.
