Technical Data

039719
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_006187.2
Gene ID
5093
Gene #
PCBP1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PCBP1, ID (PCBP1, Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3)
PCBP1, Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 187-217 amino acids selected from the Central region of human PCBP1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Cloke, B., et al. Endocrinology 151(8):3954-3964(2010)|Wang, H., et al. Cancer Cell 18(1):52-62(2010)|Zhang, T., et al. Mol. Cancer 9, 72 (2010) :|Waggoner, S.A., et al. J. Biol. Chem. 284(14):9039-9049(2009)|Huo, L.R., et al. Biochim. Biophys. Acta 1784(11):1524-1533(2008)
USBio References
No references available
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