Technical Data

039805
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Accession #
NP_000431.2
Gene ID
5145
Gene #
PDE6A
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PDE6A, ID (PDE6A, PDEA, Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha, PDE V-B1)
PDE6A, PDEA, Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha, PDE V-B1

This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 308-336 in the central region of human PDE6A, UniProt Accession #NP_000431.2; P16499|Species sequence homology: mouse and bovine.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human PDE6A.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Clark, G.R., et al. Ophthalmology 117(11):2169-2177(2010) 2.Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 3. Perlis, R.H., et al. Biol. Psychiatry 67(11):1110-1113(2010) 4. Bazhin, A.V., et al. Cell. Mol. Life Sci. 67(5):817-828(2010) 5. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
USBio References
No references available
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