Technical Data

039955
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Accession #
NP_006614; O43175
Gene ID
26227
Gene #
PHGDH
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PHGDH, ID (PHGDH, PGDH3, D-3-phosphoglycerate dehydrogenase)
PHGDH, PGDH3, D-3-phosphoglycerate dehydrogenase

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human PHGDH.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Du, H., et al. Reproduction 139(1):237-245(2010)|Burton, R.L., et al. Biochemistry 48(22):4808-4815(2009)|Kim, J.W., et al. Psychiatr. Genet. 19 (3), 161 (2009) :|Tabatabaie, L., et al. Hum. Mutat. 30(5):749-756(2009)
USBio References
No references available
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