Rabbit Anti-PHLPP2, CT (PHLPP2, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like)
PHLPP2, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like
PHLPP2 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1. This protein regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decrease cell proliferation. It also controls the phosphorylation of AKT3.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PHLPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1301-1331 amino acids from the C-terminal region of human PHLPP2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human PHLPP2
