Technical Data

039975
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Accession #
NP_056970.1
Gene ID
51050
Gene #
PI15
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PI15, NT (PI15, CRISP8, P25TI, Peptidase inhibitor 15, 25kD trypsin inhibitor, Cysteine-rich secretory protein 8, SugarCrisp)
PI15, CRISP8, P25TI, Peptidase inhibitor 15, 25kD trypsin inhibitor, Cysteine-rich secretory protein 8, SugarCrisp

This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq].

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PI15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-63 amino acids from the N-terminal region of human PI15.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008) :|Kaplan, F., et al. Am. J. Physiol. 276 (6 PT 1), L1027-L1036 (1999) :|Yamakawa, T., et al. Biochim. Biophys. Acta 1395(2):202-208(1998)|Yamakawa, T., et al. Biochim. Biophys. Acta 1395(2):202-208(1998)|Koshikawa, N., et al. J. Biochem. 119(2):334-339(1996)
USBio References
No references available
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