040021
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
HuGene ID
51588Gene #
PIAS4Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PIASy, NT (PIAS4, PIASG, E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma)
PIAS4, PIASG, E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma
PIASy1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway, the wnt pathway and the steroid hormone signaling pathway. PIASy1 is involved in gene silencing, and promotes PARK7 sumoylation.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PIASy antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56~86 amino acids from the N-terminal region of human PIASy1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Imoto, S., et al., J. Biol. Chem. 278(36):34253-34258 (2003).|Chun, T.H., et al., Circ. Res. 92(11):1201-1208 (2003).|Subramanian, L., et al., J. Biol. Chem. 278(11):9134-9141 (2003).|Liu, B., et al., Proc. Natl. Acad. Sci. U.S.A. 98(6):3203-3207 (2001).|Liu, B., et al., Proc. Natl. Acad. Sci. U.S.A. 95(18):10626-10631 (1998).USBio References
No references available