040088
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_071763.2; NP_660095.1Gene ID
64219Gene #
PJA1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PJA1, NT (PJA1, RNF70, E3 ubiquitin-protein ligase Praja-1, RING finger protein 70)
PJA1, RNF70, E3 ubiquitin-protein ligase Praja-1, RING finger protein 70
This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human PJA1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Wieland, I., et al. Clin. Genet. 72(6):506-516(2007)|Saha, T., et al. Oncogene 25(5):693-705(2006)|Mishra, L., et al. Cancer Biol. Ther. 4(7):694-699(2005)|Sasaki, A., et al. J. Biol. Chem. 277(25):22541-22546(2002)|Yu, P., et al. Genomics 79(6):869-874(2002)USBio References
No references available