Technical Data

040093
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Gene ID
23683
Gene #
PRKD3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PKC nu (PRKD3, EPK2, PRKCN, Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu)
PRKD3, EPK2, PRKCN, Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Immunofluorescence: 1:500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361~390 amino acids from the center region of human PKC nu.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Yeaman, C., et al., Nat. Cell Biol. 6(2):106-112 (2004).|Rey, O., et al., J. Biol. Chem. 278(26):23773-23785 (2003).|Matthews, S.A., et al., J. Biol. Chem. 278(11):9086-9091 (2003).|Bennasser, Y., et al., Virology 303(1):174-180 (2002).|Bennasser, Y., et al., FASEB J. 16(6):546-554 (2002).
USBio References
No references available
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