040094
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
HuGene ID
5590Gene #
PRKCZShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PKC zeta, NT (PRKCZ, PKC2, Protein kinase C zeta type, nPKC-zeta)
PRKCZ, PKC2, Protein kinase C zeta type, nPKC-zeta
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a constitutive kinase activity which is independent of calcium, and PKC activators, phosphatidylserine and diacylglycerol. Furthermore, it is insensitive to PKC inhibitors and cannot be activated by phorbol ester. The structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PKC zeta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174~203 amino acids from the N-terminal region of PKC zeta.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Li, Y.F., et al., World J. Gastroenterol. 9(9):2078-2082 (2003).|Minami, T., et al., J. Biol. Chem. 278(9):6976-6984 (2003).|Beeson, M., et al., Diabetes 52(8):1926-1934 (2003).|Laudanna, C., et al., Lab. Invest. 83(8):1155-1163 (2003).|Dada, L.A., et al., J. Clin. Invest. 111(7):1057-1064 (2003).USBio References
No references available