Technical Data

040181
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Accession #
NP_000293.2
Gene ID
5351
Gene #
PLOD1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PLOD1, NT (PLOD1, LLH, PLOD, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1, Lysyl hydroxylase 1)
PLOD1, LLH, PLOD, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1, Lysyl hydroxylase 1

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of human PLOD1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :|Huang, Q.Y., et al. Bone 44(5):984-988(2009)|Yamada, Y., et al. Int. J. Mol. Med. 19(5):791-801(2007)|Tasker, P.N., et al. Osteoporos Int 17(7):1078-1085(2006)|Giunta, C., et al. Mol. Genet. Metab. 86 (1-2), 269-276 (2005) :
USBio References
No references available
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