Rabbit Anti-PNCK, CT (PNCK, Calcium/calmodulin-dependent protein kinase type 1B, CaM kinase I beta, Pregnancy up-regulated non-ubiquitously-expressed CaM kinase)
PNCK, Calcium/calmodulin-dependent protein kinase type 1B, CaM kinase I beta, Pregnancy up-regulated non-ubiquitously-expressed CaM kinase
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Applications
Suitable for use in Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PNCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314~343 amino acids from the C-terminal region of human PNCK.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
