Rabbit Anti-POU2AF1, NT (POU2AF1, OBF1, POU domain class 2-associating factor 1, B Cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1) (FITC)
POU2AF1, OBF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1
POU2AF1 is transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry (Not Tested) and FLISA. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-27 in the N-terminal region of human POU2AF1.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human POU2AF1.
