040552
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_683877.1; NP_002777.1Gene ID
5682Gene #
PSMA1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PSMA1, CT (PSMA1, HC2, NU, PROS30, PSC2, Proteasome subunit alpha type-1, 30kD prosomal protein, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain)
PSMA1, HC2, NU, PROS30, PSC2, Proteasome subunit alpha type-1, 30kD prosomal protein, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Applications
Suitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 234-262 in the C-terminal region of human PSMA1, UniProt Accession #P25786.|Species Sequence Homology: bovine and monkey
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes human PSMA1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Zhang, Y., et al. Biochem. Biophys. Res. Commun. 355(1):245-251(2007). 2. Apcher, G.S., et al. FEBS Lett. 569 (1-3), 211-216 (2004). 3. Jayarapu, K., et al. Biochem. Biophys. Res. Commun. 314(2):523-528(2004). 4. Apcher, G.S., et al. FEBS Lett. 553 (1-2), 200-204 (2003). 5. Huang, X., et al. J. Mol. Biol. 323(4):771-782(2002)USBio References
No references available