Technical Data

040570
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_002804.2; O00233
Gene ID
5715
Gene #
PSMD9
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PSMD9, CT (PSMD9, 26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27)
PSMD9, 26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PSMD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-205 amino acids from the C-terminal region of human PSMD9.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Gragnoli, C. J. Cell. Physiol. 223(1):1-5(2010)|Huang, L.W., et al. Pathol. Oncol. Res. 16(1):81-86(2010)|Gragnoli, C. J. Cell. Physiol. 222(2):265-267(2010)|Kaneko, T., et al. Cell 137(5):914-925(2009)|Kim, J., et al. Clin. Cancer Res. 15(1):81-90(2009)
USBio References
No references available
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