040666
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_001035059.1; NP_008837.1Gene ID
84867Gene #
PTPN5Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PTPN5, NT (PTPN5, Tyrosine-protein phosphatase non-receptor type 5, Neural-specific protein-tyrosine phosphatase, Striatum-enriched protein-tyrosine phosphatase)
PTPN5, Tyrosine-protein phosphatase non-receptor type 5, Neural-specific protein-tyrosine phosphatase, Striatum-enriched protein-tyrosine phosphatase
STEP (Protein-tyrosine phosphatase striatum-enriched) is a neural-specific protein-tyrosine phosphatase. It is also known as PTPN5 (tyrosine-protein phosphatase, non-receptor type 5). STEP immunoreactive areas are most intense in the regions of the CNS receiving a dopaminergic input (e.g. striatum, basal ganglia). STEP is part of the NMDA receptor (R) complex and inhibits NMDAR single-channel activity and NMDAR-mediated synaptic current. Phosphorylation of STEP, via D1 and PKA activation by dopamine, decreases the activity of STEP by reducing its affinity for substrate.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PTPN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PTPN5.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Kurup, P., et al. J. Neurosci. 30(17):5948-5957(2010)|Rose, J. Phd, et al. Mol. Med. (2010) In press :|Korff, S., et al. BMC Cancer 8, 329 (2008) :|Eswaran, J., et al. Biochem. J. 395(3):483-491(2006)|Nguyen, T.H., et al. J. Biol. Chem. 277(27):24274-24279(2002)USBio References
No references available