Technical Data

040697-APC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
APC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Accession #
NP_056295.1
Gene ID
25945
Gene #
PVRL3
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Rabbit Anti-PVRL3, CT (PVRL3, PRR3, Poliovirus receptor-related protein 3, CDw113, Nectin-3, CD113) (APC)
PVRL3, PRR3, Poliovirus receptor-related protein 3, CDw113, Nectin-3, CD113

Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.

Applications
Suitable for use in Western Blot and FLISA. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 507-536 in the C-terminal region of human PVRL3.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human PVRL3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010. 2. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010). 3. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 4. Yu, X., et al. Nat. Immunol. 10(1):48-57(2009). 5. Fujito, T., et al. J. Cell Biol. 171(1):165-173(2005)
USBio References
No references available
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