Technical Data

040834
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_036426.3
Gene ID
9771
Gene #
RAPGEF5
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RAPGEF5, CT (RAPGEF5, GFR, KIAA0277, MRGEF, Rap guanine nucleotide exchange factor 5, Guanine nucleotide exchange factor for Rap1, M-Ras-regulated Rap GEF, Related to Epac)
RAPGEF5, GFR, KIAA0277, MRGEF, Rap guanine nucleotide exchange factor 5, Guanine nucleotide exchange factor for Rap1, M-Ras-regulated Rap GEF, Related to Epac

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RAPGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528-555 amino acids from the C-terminal region of human RAPGEF5.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010)|Hwang, S.J., et al. BMC Med. Genet. 8 SUPPL 1, S10 (2007) :|Rebhun, J.F., et al. J. Biol. Chem. 275(45):34901-34908(2000)|de Rooij, J., et al. J. Biol. Chem. 275(27):20829-20836(2000)
USBio References
No references available
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