040855
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IF IHC WBCrossreactivity
HuAccession #
NP_001092141.1; NP_722541.1Gene ID
10235Gene #
RASGRP2Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-RASGRP2, NT (RASGRP2, CDC25L, MCG7, RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, Cdc25-like protein, F25B3.3 kinase-like protein)
RASGRP2, CDC25L, MCG7, RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, Cdc25-like protein, F25B3.3 kinase-like protein
The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RASGRP2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Nagamine, K., et al. Biol. Pharm. Bull. 33(7):1138-1142(2010)|Katagiri, K., et al. J. Biol. Chem. 279(12):11875-11881(2004)|Eto, K., et al. Proc. Natl. Acad. Sci. U.S.A. 99(20):12819-12824(2002)|Toki, S., et al. J. Comp. Neurol. 437(4):398-407(2001)|Guo, F.F., et al. J. Biol. Chem. 276(27):25568-25581(2001)USBio References
No references available