Rabbit Anti-RFC3, CT (RFC3, Replication factor C subunit 3, Activator 1 38kD subunit, Activator 1 subunit 3, Replication factor C 38kD subunit)
RFC3, Replication factor C subunit 3, Activator 1 38kD subunit, Activator 1 subunit 3, Replication factor C 38kD subunit
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa.
Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RFC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237~266 amino acids from the C-terminal region of human RFC3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
