Technical Data

041005
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_006471.2
Gene ID
10636
Gene #
RGS14
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RGS14, ID (RGS14, Regulator of G-protein signaling 14)
RGS14, Regulator of G-protein signaling 14

This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Applications
Suitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 223-251 in the central region of human RGS14.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human RGS14.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Wang, J., et al. Carcinogenesis 31(10):1755-1761(2010) 2. Kottgen, A., et al. Nat. Genet. 42(5):376-384(2010) 3. Shu, F.J., et al. Cell. Signal. 22(3):366-376(2010) 4. Dowler, E.F., et al. Biomol NMR Assign 1(1):95-97(2007) 5. Martin-McCaffrey, L., et al. Cell Cycle 4(7):953-960(2005)
USBio References
No references available
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