Technical Data

041047
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_068751.4
Gene ID
60626
Gene #
RIC8A
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RIC8A, ID (RIC8A, Synembryn-A, Protein Ric-8A)
RIC8A, Synembryn-A, Protein Ric-8A

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RIC8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-271 amino acids from the Central region of human RIC8A.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Woodard, G.E., et al. Mol. Cell. Biol. 30(14):3519-3530(2010)|Matsuoka, S., et al. Science 316(5828):1160-1166(2007)|Lim, J., et al. Cell 125(4):801-814(2006)|Nishimura, A., et al. Genes Cells 11(5):487-498(2006)|Tall, G.G., et al. J. Biol. Chem. 278(10):8356-8362(2003)
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net