Technical Data

041083
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_057581.2
Gene ID
51258
Gene #
MRPL51
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RM51, CT (MRPL51, MRP64, 39S ribosomal protein L51, mitochondrial, bMRP-64)
MRPL51, MRP64, 39S ribosomal protein L51, mitochondrial, bMRP-64

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 4p and 21q.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RM51 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the C-terminal region of human RM51.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Zhang, Z., et al. Genomics 81(5):468-480(2003)|Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)|Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :|Suzuki, T., et al. J. Biol. Chem. 276(35):33181-33195(2001)
USBio References
No references available
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