Technical Data

041110
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_078815.3
Gene ID
79589
Gene #
RNF128
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RNF128, ID (RNF128, GRAIL, E3 ubiquitin-protein ligase RNF128, Gene related to anergy in lymphocytes protein, RING finger protein 128)
RNF128, GRAIL, E3 ubiquitin-protein ligase RNF128, Gene related to anergy in lymphocytes protein, RING finger protein 128

The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 115-143 in the central region of human RNF128. Species Sequence Homology: bovine
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human RNF128.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Su, L.L., et al. J. Immunol. 183(1):438-444(2009) 2. Lin, J.T., et al. J. Immunol. 182(10):5919-5928(2009) 3. Lineberry, N., et al. J. Biol. Chem. 283(42):28497-28505(2008) 4. Egawa, S., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 295 (1), G163-G169 (2008) 5. Kostianovsky, A.M., et al. J. Immunol. 178(10):6158-6163(2007)
USBio References
No references available
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