041129
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_056250.3; NP_904355.1Gene ID
25897Gene #
RNF19AShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-RNF19A, ID (RNF19A, RNF19, E3 ubiquitin-protein ligase RNF19A, Double ring-finger protein, RING finger protein 19A, p38)
RNF19A, RNF19, E3 ubiquitin-protein ligase RNF19A, Double ring-finger protein, RING finger protein 19A, p38
The protein encoded this gene contains two RING-finger motifs and an IBR (in between RING fingers) motif. This protein is an E3 ubiquintin ligase that is localized in Lewy bodies (LBs), a characteristic neuronal inclusion in Parkinson's disease (PD) brains. This protein interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. This protein is found to bind and ubiquitylate synphilin 1 (SNCAIP), which is a interacting protein of alpha synuclein in neurons, and a major component of LB. Alternatively spliced transcript variants encoding the same protein have been reported.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RNF19A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-413 amino acids from the Central region of human RNF19A.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Huh, J.W., et al. Gene 424 (1-2), 63-70 (2008) :|Matsuoka, S., et al. Science 316(5828):1160-1166(2007)|Ishigaki, S., et al. Neurobiol. Dis. 25(2):331-341(2007)|Huang, Y., et al. J. Biol. Chem. 281(17):11610-11617(2006)|Ishigaki, S., et al. J. Biol. Chem. 279(49):51376-51385(2004)USBio References
No references available