Technical Data

041152
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu
Gene ID
9025
Gene #
RNF8
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RNF8 (RNF8, KIAA0646, E3 ubiquitin-protein ligase RNF8, RING finger protein 8)
RNF8, KIAA0646, E3 ubiquitin-protein ligase RNF8, RING finger protein 8

RNF8 contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RNF8 antibody is generated from rabbits immunized with recombinant protein of human RNF8.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Noon, A.T., et al. Nat. Cell Biol. 12(2):177-184(2010)|Ramachandran, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):809-814(2010)|Marteijn, J.A., et al. J. Cell Biol. 186(6):835-847(2009)|Gong, Z., et al. J. Biol. Chem. 284(11):7284-7293(2009)|Zhang, S., et al. Cell Cycle 7(21):3399-3404(2008)
USBio References
No references available
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