041217
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_000962.2Gene ID
6129Gene #
RPL7Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-RPL7, NT (RPL7, 60S ribosomal protein L7)
RPL7, 60S ribosomal protein L7
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RPL7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of human RPL7.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Chou, C.W., et al. FEBS Lett. 584(19):4151-4156(2010)|Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)|Robledo, S., et al. RNA 14(9):1918-1929(2008)|Wu, W.C., et al. FEBS Lett. 581(4):651-657(2007)USBio References
No references available