Technical Data

041310
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
64432
Gene #
MRPS25
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-RT25, CT (MRPS25, RPMS25, 28S ribosomal protein S25, mitochondrial)
MRPS25, RPMS25, 28S ribosomal protein S25, mitochondrial

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144~173 amino acids from the C-terminal region of human RT25.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :|Zhang, Z., et al. Genomics 81(5):468-480(2003)|Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :|Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)|Hattori, A., et al. DNA Res. 7(6):357-366(2000)
USBio References
No references available
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