041379-PE
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
PEIsotype
IgGGrade
Affinity PurifiedApplications
FLISA IHC WBCrossreactivity
HuAccession #
NP_112222.1Gene ID
81833Gene #
SPACA1Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
Rabbit Anti-SACA1, NT (SPACA1, SAMP32, Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32) (PE)
SPACA1, SAMP32, Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from infertile males. Furthermore, antibodies generated against the recombinant protein block in vitro fertilization. This protein localizes to the acrosomal membrane of spermatids and mature spermatozoa where it is thought to play a role in acrosomal morphogenesis and in sperm-egg binding and fusion, respectively.
Applications
Suitable for use in FLISA, Western Blot and Immunohistochemisty. Other potential application, though not tested is Direct Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 46-74 in the N-terminal region of human SACA1.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human SACA1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) 2. Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007). 3. Vasan, R.S., et al. BMC Med. Genet. 8 SUPPL 1, S2 (2007). 4. Mungall, A.J., et al. Nature 425(6960):805-811(2003) 5. Hao, Z., et al. Biol. Reprod. 66(3):735-744(2002)USBio References
No references available