Technical Data

041461
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_057594.3; Q96I5
Gene ID
51540
Gene #
SCLY
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SCLY, NT (SCLY, SCL, Selenocysteine lyase)
SCLY, SCL, Selenocysteine lyase

Selenocysteine lyase (SCLY; EC 4.4.1.16) catalyzes the pyridoxal 5-prime phosphate-dependent conversion of L-selenocysteine to L-alanine and elemental selenium (Mihara et al., 2000 [PubMed 10692412]).

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 8-36 in the N-terminal region of human SCLY, UniProt Accession #NP_057594.3; Q96I5.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SCLY.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 2. Yepes, J.O., et al. Biomedica 26(2):194-205 (2006). 3. Mihara, H., et al. J. Biol. Chem. 275(9):6195-6200 (2000). 4. Daher, R., et al. J Trace Elem Electrolytes Health Dis 6(3):189-194 (1992). 5. Esaki, N., et al. J. Biol. Chem. 257(8):4386-4391 (1982)
USBio References
No references available
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