Technical Data

041597
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_653266.2
Gene ID
143686
Gene #
SESN3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Sestrin-3, ID (Y335) (SESN3, SEST3, Sestrin-3)
SESN3, SEST3, Sestrin-3

Sestrins form a small family of redox enzymes which regulate accumulation of Reactive Oxygen Species. They protect cells and their DNA against oxidative damage and regulate cell growth and viability. These genes are often deregulated in cancers and their inactivation accelerates the growth of model tumors in mice.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Sestrin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-347 amino acids from the Central region of human Sestrin-3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Chen, C.C., et al. Dev. Cell 18(4):592-604(2010)|Peeters, H., et al. Hum. Genet. 112 (5-6), 573-580 (2003) :
USBio References
No references available
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Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net