Rabbit Anti-SGEF, ID (ARHGEF26, SGEF, Rho guanine nucleotide exchange factor 26, SH3 domain-containing guanine exchange factor)
ARHGEF26, SGEF, Rho guanine nucleotide exchange factor 26, SH3 domain-containing guanine exchange factor
SGEF activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and promotes bacterial entry.
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 378-406 in the central terminal region of human SGEF.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography..
Specificity
Recognizes human SGEF.
