Technical Data

041749
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo
Accession #
NP_001103820.1
Gene ID
20466
Gene #
Sin3a
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Sin3a, CT (Sin3a, Kiaa4126, Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a)
Sin3a, Kiaa4126, Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a

Sin3a acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1078-1106 in the C-terminal region of mouse Sin3a. Species Sequence Homology: human
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes mouse Sin3a

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Baltus, G.A., et al. J. Biol. Chem. 284(11):6998-7006(2009). 2. Anderson, D.M., et al. Dev. Dyn. 238(3):572-580(2009). 3. Chang, S., et al. J. Immunol. 181(12):8372-8381(2008). 4. van Oevelen, C., et al. Mol. Cell 32(3):359-370(2008). 5. Wilkinson, D.S., et al. Mol. Cell. Biol. 28(6):1988-1998(2008)
USBio References
No references available
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