Technical Data

041769
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_872426.1
Gene ID
348235
Gene #
SKA2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SKA2, NT (SKA2, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A)
SKA2, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.

Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within aa9-37 from N terminal region of human SKA2. Species Sequence Homology: monkey
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes human SKA2

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Cao, G., et al. Biochem. Biophys. Res. Commun. 396(4):978-982(2010). 2. Welburn, J.P., et al. Dev. Cell 16(3):374-385(2009). 3. Rice, L., et al. J. Endocrinol. 198(3):499-509(2008). 4. Lamesch, P., et al. Genomics 89(3):307-315(2007). 5. Hanisch, A., et al. EMBO J. 25(23):5504-5515(2006)
USBio References
No references available
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