Technical Data

041794
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_006589.2
Gene ID
10723
Gene #
SLC12A7
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SLC12A7, NT (SLC12A7, KCC4, Solute carrier family 12 member 7, Electroneutral potassium-chloride cotransporter 4, K-Cl cotransporter 4)
SLC12A7, KCC4, Solute carrier family 12 member 7, Electroneutral potassium-chloride cotransporter 4, K-Cl cotransporter 4

SLC12A7 mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).

Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 89-117 in the N-terminal region of human SLC12A7.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human SLC12A7

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hartmann, A.M., et al. J. Biol. Chem. 285(31):23994-24002(2010). 2. Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010). 3. Chen, Y.F., et al. Cancer Res. 69(22):8585-8593(2009). 4. Fujii, T., et al. J. Biol. Chem. 284(1):619-629(2009). 5. Ji, W., et al. Nat. Genet. 40(5):592-599(2008)
USBio References
No references available
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