Technical Data

042103
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_073716.1; NP_073717.1
Gene ID
6638
Gene #
SNRPN
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SNRPN, NT (SNRPN, HCERN3, SMN, Small nuclear ribonucleoprotein-associated protein N, Sm protein D, Sm protein N, Tissue-specific-splicing protein)
SNRPN, HCERN3, SMN, Small nuclear ribonucleoprotein-associated protein N, Sm protein D, Sm protein N, Tissue-specific-splicing protein

The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing, possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
SNRPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human SNRPN.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Benetatos, L., et al. Leuk. Res. 34(2):148-153(2010)|Kim, S.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (7), 1116-1125 (2008) :|Horsthemke, B., et al. Am. J. Med. Genet. A 146A (16), 2041-2052 (2008) :|Runte, M., et al. Hum. Mol. Genet. 10(23):2687-2700(2001)|Kuslich, C.D., et al. Am. J. Hum. Genet. 64(1):70-76(1999)
USBio References
No references available
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