Technical Data

042230
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_003112.2
Gene ID
6689
Gene #
SPIB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SPIB, CT (SPIB, Transcription factor Spi-B)
SPIB, Transcription factor Spi-B

SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
SPIB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the C-terminal region of human SPIB.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Liu, X., et al. Nat. Genet. 42(8):658-660(2010)|Schmidlin, H., et al. Blood 112(5):1804-1812(2008)|Nagasawa, M., et al. Eur. J. Immunol. 38(9):2389-2400(2008)|Dontje, W., et al. Blood 107(6):2446-2452(2006)|Geng, C.D., et al. J. Biol. Chem. 280(52):43264-43271(2005)
USBio References
No references available
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