Technical Data

042319
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001122325.1; NP_001122324.1
Gene ID
51593
Gene #
SRRT
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SRRT, NT (SRRT, ARS2, ASR2, Serrate RNA effector molecule homolog, Arsenite-resistance protein 2)
SRRT, ARS2, ASR2, Serrate RNA effector molecule homolog, Arsenite-resistance protein 2

SRRT acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and RNASEN, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
SRRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-81 amino acids from the N-terminal region of human SRRT.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Eijgelsheim, M., et al. Hum. Mol. Genet. 19(19):3885-3894(2010)|Kiriyama, M., et al. Mol. Cell. Biol. 29(17):4729-4741(2009)|Gruber, J.J., et al. Cell 138(2):328-339(2009)|Wilson, M.D., et al. Mol. Cell. Biol. 28(5):1503-1514(2008)|Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
USBio References
No references available
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