Technical Data

042327
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IF IHC WB
Crossreactivity
Hu
Accession #
NP_060327.3;
Gene ID
54961
Gene #
SSH3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SSH3, CT (SSH3, SSH3L, Protein phosphatase Slingshot homolog 3, SSH-like protein 3)
SSH3, SSH3L, Protein phosphatase Slingshot homolog 3, SSH-like protein 3

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50 Immunofluorescence: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of human SSH3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kousaka, K., et al. Genesis 46(5):246-255(2008)|Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007)|Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)|Soosairajah, J., et al. EMBO J. 24(3):473-486(2005)|Ohta, Y., et al. Genes Cells 8(10):811-824(2003)
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net