Technical Data

042512
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_035648.2
Gene ID
20963
Gene #
Syk
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Syk, ID (Syk, ptk72, Sykb, Tyrosine-protein kinase SYK, Spleen tyrosine kinase)
Syk, ptk72, Sykb, Tyrosine-protein kinase SYK, Spleen tyrosine kinase

Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion. Phosphorylates USP25 and regulates its intracellular levels (By similarity).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse Syk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-223 amino acids from the Central region of Mouse Syk.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Heizmann, B., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18563-18568(2010)|Fernandez, P.L., et al. J. Biol. Chem. 285(43):32844-32851(2010)|Zou, W., et al. J. Cell. Sci. 123 (PT 17), 2955-2963 (2010) :|Falet, H., et al. J. Exp. Med. 207(9):1967-1979(2010)|Dierks, C., et al. Cancer Res. 70(15):6193-6204(2010)
USBio References
No references available
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Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net