Technical Data

042681
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_006747.1; NP_958845.1
Gene ID
6917
Gene #
TCEA1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TCEA1, ID (TCEA1, GTF2S, TFIIS, Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIIS.o)
TCEA1, GTF2S, TFIIS, Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIIS.o

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TCEA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-126 amino acids from the Central region of human TCEA1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)|Matsuoka, S., et al. Science 316(5828):1160-1166(2007)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Asp, J., et al. Genes Chromosomes Cancer 45(9):820-828(2006)
USBio References
No references available
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