Rabbit Anti-TCEB1, CT (TCEB1, Transcription elongation factor B polypeptide 1, Elongin 15kD subunit, Elongin-C, RNA polymerase II transcription factor SIII subunit C, SIII p15)
TCEB1, Transcription elongation factor B polypeptide 1, Elongin 15kD subunit, Elongin-C, RNA polymerase II transcription factor SIII subunit C, SIII p15
This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.
Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 69-98 in the C-terminal region of human TCEB1, UniProt Accession #NP_005639.1; Q15369; P83941; P83940; Q2KII4.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human TCEB. Species Sequence Homology: bovine, mouse and rat
