Technical Data

042693
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_996923.1; NP_996919.1
Gene ID
6938
Gene #
TCF12
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TCF12, NT (TCF12, BHLHB20, HEB, HTF4, Transcription factor 12, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4)
TCF12, BHLHB20, HEB, HTF4, Transcription factor 12, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TCF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-84 amino acids from the N-terminal region of human TCF12.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Schotte, R., et al. J. Immunol. 184(12):6670-6679(2010)|Rose, J. Phd, et al. Mol. Med. (2010) In press :|Landa, I., et al. PLoS Genet. 5 (9), E1000637 (2009) :|Gardini, A., et al. PLoS Genet. 4 (11), E1000275 (2008) :|Kumar, R., et al. J. Biol. Chem. 283(27):19026-19038(2008)
USBio References
No references available
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