Technical Data

042779
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu
Accession #
NP_000349.1
Gene ID
7045
Gene #
TGFBI
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TGFBI, NT (TGFBI, BIGH3, Transforming growth factor-beta-induced protein ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein)
TGFBI, BIGH3, Transforming growth factor-beta-induced protein ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 106-135 amino acids from the N-terminal region of human TGFBI. Species Sequence Homology: bovine, mouse, porcine
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human TGFBI.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 2. Edelstein, S.L., et al. Cornea 29(6):698-700(2010) 3. Romero, P., et al. Mol. Vis. 16, 1601-1609 (2010). 4. Paliwal, P., et al. Mol. Vis. 16, 1429-1438 (2010) 5. Yang, J., et al. Mol. Vis. 16, 1186-1193 (2010) :
USBio References
No references available
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