Technical Data

043315
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
DB E
Crossreactivity
Mo
Accession #
NP_075025.2
Gene ID
64930
Gene #
Tsc1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TSC1, phosphorylated (S312) (Tsc1, Kiaa0243, Hamartin, Tuberous sclerosis 1 protein homolog)
Tsc1, Kiaa0243, Hamartin, Tuberous sclerosis 1 protein homolog

In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity).

Applications
Suitable for use in Dot Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Dot blot 1:500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
mouse TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S312 of mouse TSC1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kladney, R.D., et al. Cancer Res. 70(21):8937-8947(2010)|Sathaliyawala, T., et al. Immunity 33(4):597-606(2010)|Scott, C.L., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 299 (4), L455-L471 (2010) :|Bartolome, A., et al. Endocrinology 151(7):3084-3094(2010)|Squarize, C.H., et al. PLoS ONE 5 (5), E10643 (2010) :
USBio References
No references available
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