Technical Data

043434
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_003309.2
Gene ID
7272
Gene #
TTK
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TTK, NT (TTK, MPS1, MPS1L1, Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase)
TTK, MPS1, MPS1L1, Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase

This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of human TTK.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Cui, Y., et al. J. Biol. Chem. 285(43):32988-32998(2010)|Maciejowski, J., et al. J. Cell Biol. 190(1):89-100(2010)|Santaguida, S., et al. J. Cell Biol. 190(1):73-87(2010)|Hewitt, L., et al. J. Cell Biol. 190(1):25-34(2010)|Lan, W., et al. J. Cell Biol. 190(1):21-24(2010)
USBio References
No references available
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