Technical Data

043435
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_714923.1
Gene ID
150465
Gene #
TTL
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TTL, CT (TTL, Tubulin-tyrosine ligase)
TTL, Tubulin--tyrosine ligase

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-385 amino acids from the C-terminal region of human TTL.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :|Erck, C., et al. Cytogenet. Genome Res. 101(1):47-53(2003)|Mialhe, A., et al. Cancer Res. 61(13):5024-5027(2001)|Ersfeld, K., et al. J. Cell Biol. 120(3):725-732(1993)
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net